2/5 apart, not needed o Otherwise, ensure cutoff is 10nm less than desired emission wavelength. Prismatic colour and motion for everyone. Increased fluorescence performance. Nucleic Acid and Protein Quantitation. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Spectranet customer portalThe SpectraMax Paradigm Multi-Mode Detection Platform is a modular multi-mode microplate detector. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. 304 signal level saturation. Review date: 19 Mar 2018 | SpectraMax i3x Multi-Mode Microplate Reader with MiniMax Imaging Cytometer The SpectraMax ® line of user-friendly microplate readers provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and moreSimilar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. Prepare your i3/i3x instrument for shipment. 1 mg/mL density of resazurin, protected of the light, incubated for 1 h at room temperature. Anne T. Using the SpectraMax i3x reader, the preconfigured SpectraMax Glo Steady-Luc Reporter assay protocol in SoftMax Pro Software was used to measure luminescence. 19 Switch on the SpectraMax i3x plate reader and launch SoftMax Pro software. 7°C (gradient heating design). Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. In SoftMax® Pro, the following Automix settings are available for the SpectraMax i3/i3x Instrument: Intensity : Low, Medium, or High. 발광 (Luminescence) 리더기. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,*On the SpectraMax i3x reader only. The corrected absorbance is within 5% of the. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. applications, including dual luciferase and ATP assays. The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3, i3x and Paradigm Multi-Mode Readers. SpectraMax i3x 多功能微孔板读板机 SpectraMax Paradigm 多功能微孔板读板机 SpectraMax M5e 多功能微孔板读板机 扫一扫关注我 们的官方微信 Molecular Devices 大中华区 全国咨询服务热线: 400-820-3586 Email: info. 0. 58% for the detection module and 2. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. Bookings are free, but you will need to use iLab to book. The UV-Vis spectroscopy was measured on a TU-1900Beijing PuXi instrument. The SoftMax Pro Software provides integrated instrument control. User-upgradeable applications The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 9 x 10-15. ZERO BIAS - scores, article reviews, protocol conditions and moreWe would like to show you a description here but the site won’t allow us. In this product video, see how the SpectraMax M5 quickly reads samples. BRET2canbeusedtostudyvariouskindsofprotein-proteininteractions. Order Now. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. X-ray powder diffractometer (XRD) with Cu- Ka radiation (Bruker AXS D8) was used to identify the crystalline phase of as synthesized C-AuNPs. Figure 1. Reduce background noise The cooled PMT reduces background noise allowing for a more sensitive, wide dynamic range in extremely low light. The user guide also covers the features and specifications of the SpectraMax MiniMax 300. Bioz Stars score: 98/100, based on 1 PubMed citations. SpectraMax i3x Multi-Mode Detection Platform. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. The ratio of the two fluorescence intensities 520/620 (acceptor/donor) enables the calculation of Delta F (%) which. Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. Danaher Inc spectramax i3x. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. *On the SpectraMax i3x reader only. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Easily upgrade or switch between detection modes with user-exchangeable modules – just open the drawer and insert the module you want. The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. CRITICAL STEP Avoid air bubbles; reverse pipetting the luciferase reagent into the plate is a good way to achieve this. SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. The Relative Luminescence Units (RLU) were measured using the SpectraMax i3x Luminescence Glow cartridge (Lum 384) (0200-7015POS, Molecular Devices) and normalized to the cell count or area, which were determined using the Mini MaxTM 300 Imaging Cytometer (5024062, Molecular Devices). Because a PMT needs to be set to a specified gain to reach a specific sensitivity,Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. Singlet oxygen (1 O 2) generation was determined through 1,3-diphenylisobenzofuran (DPBF) quenching. As. g. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. We would like to show you a description here but the site won’t allow us. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. 0001 0. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. We would like to show you a description here but the site won’t allow us. The absorbance at 645 nm was subsequently determined using a Spectra Max i3x microplate reader (Molecular Devices). INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellSpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. 2. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. Get Pricing. Get a front row view of your cells. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. Learn more about its features, specifications, and applications in this user guide. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. MD Neurotransmitter Transporter Assay. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Dilution series of HeLa cells transfected with firefly (red plot) and Renilla (blue plot) luciferases. 99. The SpectraMax i3x reader provides theThe SpectraMax® i3x Multi-Mode Microplate Reader with the SpectraMax® MiniMax™ 300 Imaging Cytometer can be used to image slides using a specialized slide adapter. ，相关视频：多功能酶标仪SpectraMax i3x的维护操作-注射器卡盒，多功能酶标仪SpectraMax iD5的维护操作-注射器卡盒，多功能酶标仪SpectraMax i3x的维护操作-卡盒的安装和拆卸，细胞计数及活率分析仪 Vi-CELL BLU 功能&操作讲解，CRISPR基因编辑系统介绍：Cas9和Cas12a，流式. 11. D. The FRET ratio is defined as the relative fluorescence intensity of an acceptor normalized to the total fluorescence intensity. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the dataBased on the SpectraMax i3x system, ACROBiosystems provides biomedical research and development services for customers seeking quantification and detection of antibodies-antigen interaction. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Prepare your i3/i3x instrument for shipment. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strengthTNF-alpha quantification down to low picomolar levels on the SpectraMax Paradigm Platform. We would like to show you a description here but the site won’t allow us. 1. 0. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user-configurable. 02, 0. The SpectraMax ® i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Patented user-exchangeable cartridge design expands the system's detection capability making it. Introduction. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. i3. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Multimode Readers Comparison Chart. Next,. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Multimode Reader Comparison Chart. 316 grating motor driver faulty. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. The chemical reduction of resazurin was evaluated incubating each well of the microtiter plate with 10 μL (10% of final well volume) of 0. Generate more data points without the need to dilute. Its dual light sources, a xenon lamp and LEDs, illuminates samples with incomparable signal strength and superior sensitivity. Published Dec 16, 2020 | Updated Dec 16, 2020. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. ATP (“flash” luminescence using . 0´ 105 dsD NA (p g/ wel ) R F USpectraMax Glo Steady-Luc 工作液都加 入到微孔板后，震荡摇匀并避光放置 10 min。使用 SpectraMax Glo Steady-Luc Reporter 内置的 SpectraMax Glo Steady- Luc Reporter 实验模板设定程序并通过 SpectraMax i3x 读板机检测发光。检测结 束后，模板会根据数据自动生成一条曲 线。SpectraMax MiniMax 300 细胞成像系统 为 SpectraMax i3x 多功能微孔板检测平台而打造！ • • • • 主要特点 系统全面兼容细胞成像 及微孔板检测技术 独特无标记分析技术无需 对细胞染色即可进行细胞 计数和贴壁度的计算 具有多种检测功能可对 细胞活力及毒性进行全. Examples: fiPress carefully to engage,fl fiDo not press down. Measure oxidative metabolism and glycolytic activity on the SpectraMax i3x reader. The SpectraMax i3x Injector Cartridge with SmartInject Technology is a data sheet that describes the features and benefits of this innovative accessory for the SpectraMax i3x Multi-Mode Detection Platform. Italic and boldface type are used for emphasis. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strength and superior sensitivity across the full. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Measured using the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. Products : SoftMax Pro Software, SoftMax Pro GxP Software, CellReporterXpress software, General, Screenworks, pCLAMP/AxoScope, GenePix Pro, MetaXpress software. , 2014; Xiong et al. The plate was covered and incubated for one hour at room temperature. Transfected cells were imaged on the SpectraMax MiniMax 300 Imaging Cytometer. Here, we demonstrate the benefits of using the SpectraMax ® i3x Multi-Mode Microplate Reader with the SpectraMax ® MiniMax™ 300 Imaging Cytometer in combination with MetaMorph ® Microscopy Automation and Image Analysis Software for performing automated neurite outgrowth imaging and analysis. User-installable and removable detection cartridges allow the detector to be configured for specific applications and easily. Data Analysis. Relative mitochondrial polarization was expressed as the ratio of emission at 590 nm to that at 530 nm (Shin et al. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Designed specifically for the SpectraMax® i3 Multi-Mode Detection Platform, the field-upgradeable SpectraMax® MiniMax™ 300 Imaging Cytometer enables quick inspection of cells and provides insight into phenotypic changes that are important for understanding cytotoxicity, cell proliferation, and protein expression. Multi-Mode Reader. Specifically, we present neurite. Recommended settings for SpectraMax iD5 and i3x readers. 0 mm hex key. 6 in. SpectraMax i3x offers all the techniques you need to explore cell pathway in a single system. 4 / 5. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. Page 9 Installing and Setting Up the SpectraMax MiniMax Imaging Cytometer Attaching the Imaging Cytometer to the Microplate Reader Attaching the SpectraMax MiniMax Imaging Cytometer to the SpectraMax i3x Instrument requires a work area that is approximately 130 cm (51. Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. Bound crystal violet was dissolved with 10% acetic acid and the optical density (OD) at 570 nm was determined using a Spectra Max i3X spectrophotometer (Molecular Devices). Turbidity measurements were performed with a Spectra Max® i3x microplate reader to study the effect of oxidation on the kinetics of sFN formation. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. It allows you to explore cellular . The SpectraMax i3x’s user-upgradeable application modules will future-proof your research to include most assay capabilities of the SpectraMax iD3 and iD5 readers, as well as additional capabilities such as imaging. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. The SpectraMax DuoLuc Reporter Assay has been performance optimized on the SpectraMax i3x reader with Injector Cartridge. The SpectraMax i2X is used for cell migration, cell. In this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). 5 decades, with sensitive detection of cells. The SpectraMax i3x reader provides theWith these results, as well as CV values below 6% for all standards, both the SpectraMax i3x and the SpectraMax iD5 readers demonstrate high sensitivity and a wide dynamic range for the IP-One assay. The SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. Features. Find regional support. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Spectranet customer portal The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. Description 1. It allows you to explore cellular pathways and protein activation and expression in one system. The SpectraMax® i3x Multi-Mode Microplate Reader with the SpectraMax® MiniMax™ 300 Imaging Cytometer is equipped with transmitted light (TL), green fluorescence (Ex/Em: 460/541), and red (625/713) fluorescence channels. the SpectraMax Paradigm Multi-Mode Detection Platform User Guide. This instrument also provides the option of modular upgrades for Western Blotting,. 1step assay with SpectraMax microplate readers. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. Promega. Download PDF. 99 to $1999. 0 | 9 reviews | Write your own review Measures Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgradesMolecular Devices SpectraMax i3x Multi-Mode Microplate Platform. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. TNF-alpha quantification down to low picomolar levels on the SpectraMax Paradigm Platform. Prepare your iD series instrument for shipment. each new assay. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. 312 gain calibration failed. Other services inlcude but are not limited to anti-drug activity (ADA), PK/PD assays as well as antibody screenings. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. SpectraMax i3/i3x readers must be equipped with a suitableWhen we measured the FRET ratio using the Spectramax i3x plate reader, we found that FRET ratios were higher in DMSO-treated cells that had not been incubated at room temperature to enhance fluorescence than in room temperature-incubated cells (Fig. 0. Spectranet customer portalWe would like to show you a description here but the site won’t allow us. SpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi. Keywords: fluorescence polarization, spectramax detection cartridge, spectramax paradigm readers, spectramax paradigm, upgredeable cartridge, Created Date: 8/1/2013 10:01:48. It allows the user to determine the total concentration of protein in a solution. Next, an AlphaLISA assay was developed to detect levels of pro-inflammatory factors produced by endothelial cells in response to TNF-alpha stimulation. Promega. ). 흡광 (Absorbance) 리더기. pathways and protein activation and expression in one system. ATP LLD (pM) ATP LLD (moles) SpectraMax i3x, luminescence . These robust readers have been placed in labs from Antarctica to the. Dual auto injector cartridges for the SpectraMax® i3x Multi-Mode Microplate Detection Platform allows you to expand your research capabilities to include flash based applications, including dual luciferase and ATP assays. 04, 0. As shown in the results above, the DELFIA cell proliferation assay combined with the SpectraMax i3 reader gives researchers a very sensitive test for measuring cell proliferation. Italic and boldface type are used for emphasis. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Thetypeof luciferase(Renilla. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. Find regional support. SoftMax Pro Software enables users to quickly set up analysis of cell counts via a simple point and click user interface. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Explore a wealth of applications with the future-ready SpectraMax i3x. HTRF-compatible SpectraMax Multi-Mode Readers. 1、卡盒式模块和细胞成像模块的构架检测功能（如FP、TRF、HTRF、AlphaScreen、ScanLater Western Blot等）卡盒模块化设计，仅通过插入卡盒就能可. The SpectraMax i3x Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. , Fisher Scienti fi c, cat. 2 (Molecular Devices, San Jose, CA). The SpectraMax i3x Injector Cartridge with SmartInject Technology is a data sheet that describes the features and benefits of this innovative accessory for the SpectraMax i3x Multi-Mode Detection Platform. Winnersh Triangle. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel detection capabilities without the need for service. Manufacturers try to design their instruments so they can be. Spectranet customer portalWatch this video to get an introduction to the Injector-Ready SpectraMax® i3x Multi-Mode Microplate Reader System. The SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. To read HTRF®, the SpectraMax i3x® must be first equipped with the SpectraMax i3x® Cisbio HTRF® cartridge, which enables the simultaneous measurement of both 620nm donor and 520 nm acceptor emissions. Modularity does provide some measure of forward vision into upcoming or future experimental needs. 306 plate air cal. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. HTRF IP1 standard curve measured on the SpectraMax i3x (green circles) and SpectraMax iD5 (red circles) readers. com. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,SpectraMax M3 Plate Read, Plate Map, Data Analysis and Export Setup pg. Microplate readers, multi-mode, SpectraMax® i3x. Unlock your i3/i3x instrument after shipment. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. SpectraMax® i3 Multi-ModeDetectionPlatform withtheSpectraMax® MiniMax™ImagingCytometer UserGuide 5025027A April2013. Itemploysa luciferaselabel(where“label”generallymeansfusedbytheuseofexpressionvectors)forthe oneproteinandaGreenFluorescentProtein(GFP)variantlabelfortheother. Microplate Stacker. It allows you to explore cellular pathways and protein activation and expression in one system. Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. The SpectraMax MiniMax 300 Imaging Cytometer option is a field-upgradeable option for the SpectraMax i3/i3x Multi-Mode Detection Platform that offers cellular imaging capability. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. Contact Local Team. no. For each assay kit, a preconfigured protocol is included in SoftMax Pro Software to facilitate the acquisition and analysis of data for a simplified workflow. User-upgradeable detection modules allow you to expand the capabilities of your microp. Analysis of HTRF assays involves Cisbio’s patented ratiometric reduction method based on the two emission wavelengths detected. This is not due to a temperature-dependent alteration in cellular autofluorescence,. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Data Analysis. The SpectraMax i3x reader is engineered to perform with Spectral Fusion Illumination for increased sensitivity across the entire wavelength range, and a cooled photomultiplier tube (PMT) for improved detection in extremely. Reaction catalyzed by the firefly luciferase. For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. 2. Quantification of NETs was performed with the SpectraMax i3x microplate-reader (Molecular Devices, United Kingdom). This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Molecular Devicesの全商品を見る. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Preconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. 3: The cuvette chamber. The plate was shaken and incubated in the dark for 10 minutes. The ABS reader ranges from 340-850 nm in wavelength and the ABS Plus reader ranges from 190-1000 nm, which is equivalent to having 510 or 810 free filters, respectively. Immunofluorescence StainingAfter cooled down to room temperature, the concentrations of the solution were measured by microplate reader (Spectra Max i3x) at 490 nm (Kagi and Schaffer, 1988). Explore a wealth of applications with the future-ready SpectraMax i3x. Description 1. 304 signal level saturation. SpectraMax i3x 多功能微孔板读板机 SpectraMax Paradigm 多功能微孔板读板机 SpectraMax M5e 多功能微孔板读板机 扫一扫关注我 们的官方微信 Molecular Devices 大中华区 全国咨询服务热线: 400-820-3586 Email: info. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. TR-FRET was measured on the SpectraMax i3x and iD5 readers (see Table 1 for instrument settings). The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. SpectraMax i3x Multi-Mode Microplate Reader System. 1. Molecular Devices, LLC Reading Modes: Endpoint and Kinetic; Optical System: Spectral Fusion Illumination. Italic and boldface type are used for emphasis. Lower limits of detection for ATP determined using the ATPlite . The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. 2. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. It allows you to explore cellular pathways and protein activation and expression in one system. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. ) deep, preferably with access on all sides. Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. SpectraMax® i3x Multi-Mode Detection Platform, Molecular Devices. Fourier transform infrared (FT-IR) spectra of cCQD were obtained on a. 315 can't find zero order. ISM Equipment Classification (Group 1, Class A) This equipment is. no. 2. 1. Find regional support. Each heating element is independently. ValitaTiter Plus settingsThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The SoftMax Pro Software provides integrated instrument control. Molecular Devices are known for both the reliability and the wide uses of their products. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideSpectraMax® Microplate Readers and SoftMax® Pro Software. 635. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. The chamber. GFP expression was higher at 21 hours post transfection than at 16 hours (16-hour data not shown). Enzyme Activation Analysis;. Item SpectraMax i3x Multi-Mode Microplate Readers; Company Molecular Devices LLC; Catalog Number SpectraMax i3x <Disabled> This product is no longer available on Biocompare. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . MTT was purchased from Sigma-Aldrich (St. 303 unable to cal dark current. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. Promega. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. Capture Flash Assays with Ease. Learn how the SpectraMax Paradigm Multi-Mode Detection Platform with Tunable Wavelength (TUNE) Detection Cartridge combines the flexibility of wavelength scanning with the high sensitivity of filters. 303 unable to cal dark current. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. The absorbance at 450 nm was read with a Spectra Max i3X microplate spectrophotometer (Molecular Devices, Sunnyvale, CA, USA), and the data at 30–40 min post-treatment were analyzed. Figure 4. The SpectraMax Injector Cartridge enables precise reagent delivery and adjustment of delay and integration times that are required to obtain optimal sensitivity for this assay. 200-800 nm using Spectra Max i3X microplate reader. Easily upgrade or switch between detection modes with user-exchangeable modules – just open the drawer and insert the module you want. Detection cartridge modularity allows for easy addition of new read modes within minutes without. Enzyme activity was presented as absolute activity in a unit of nmol g −1 dry soil h −1. Videos & Webinars SpectraMax i3x - Detection Module ChangeSpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular. SpectraMax i3, i3x, and iD5 readers. After correcting for negative controls and quench standards, the soil enzyme activities were expressed in units of nmol h −1 g −1 soil (German et al. Note: Additional readers with similar performance are listed. The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. 2 in. Patented user-exchangeable cartridge design expands the system's detection capability making it. 0 mm hex key to remove the six blue retaining screws that secure the transport bracket (B) to the instrument. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel detection capabilities without the need for service. D 1 1. Multimode Readers Comparison Chart. Microplate and read height should be optimized for each new lot of microplates or assay volume used (use the Show Pre-Read Optimization options in the instrument settings in SoftMax Pro Software). SpectraMax i3x Multi-Mode Microplate Reader; SpectraMax MiniMax 300 Imaging Cytometer *Here, the NucView 488 with DMSO formulation was used. The SpectraMax i3 is specifically used in this lab for BCA assays and Elisa Assays. SpectraMax i3x. Supplier: Molecular Devices. 0´ 104 6. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. The SpectraMax i3x reader provides the. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. 3) At the bottom of the strip, click on "Show. SpectraMax Quant dsDNA Assay Kits. The information below applies to a standard 96 well plate, set to a linear shake mode. Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. 10, and 0. 00800 665 32860. Description 1. 305 reference level saturation. i3x. The corrected absorbance is within 5% of the value. 315 can't find zero order. These concentrations are then used to perform western blots. Louis, MO) and used as received without further purification. Introduction. 800. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT.